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Figure 4 | Genome Biology

Figure 4

From: User guide for mapping-by-sequencing in Arabidopsis

Figure 4

Effect of coverage and pool size on BC 2 F 2 and BC 3 F 2 backcross populations. (A) Pools of 3 to 70 BC2F2 individuals (colored blocks) were sequenced with increasing coverage ranging from 5x to 200x. For each combination of pool size and coverage we simulated 500 independent populations and performed a mapping-by-sequencing analysis on each of them. Average number of candidate mutations with one standard deviation is shown on the y-axis. The initial number of mutations per genome was 1,400. (B) Outcome of the same analysis with BC3F2 recombinants.

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