Results of mapping-by-sequencing with backcross populations. (A) Pools of 3 to 70 BC1F2 individuals (colored blocks) were sequenced with increasing coverage ranging from 5x to 200x. For each combination of pool size and coverage we simulated 500 independent populations and performed a mapping-by-sequencing analysis on each of them. Average number of candidate mutations with one standard deviation is shown on the y-axis. The initial number of mutations per genome was 1,400. (B) Zoom in on the framed region in panel A. Pools with three recombinants are not shown.