Figure 4From: DNA methylation dynamics during intestinal stem cell differentiation reveals enhancers driving gene expression in the villusAnalysis of histone modifications and local interactions of DMRs. (a) Numbers of DMRs called between methylomes and their association with different genomic regions as indicated. A DMR is labelled as TSS if within 2 kb of an annotated transcriptional start. DMRs are labelled as intergenic if they are not within 2 kb of a refseq gene. DMRs that overlap with multiple genomic elements are counted in both categories. Top graph represents DMRs that show a decrease in DNA methylation upon differentiation, lower graph depicts the DMRs with an increase during differentiation. (b) Scatter plot displaying the absolute difference in DNA methylation of the DMRs (GFP_High - Villus) (X-axis) versus the difference in expression of the associated genes between GFP_high cells and the Villus (Y-axis) (GFP_High divided by Villus). (c-e) Chip-qPCR results for different histone modifications found in chromatin of Villus epithelium for eight individual DMRs. DMRs are labelled after their closest gene (as indicated), or after their chromosomal location. * P <0.05 (student t-test). $: 4C analysis shown in, Additional file 2, Figure S6. The control is a randomly chosen region close to the Sp5 TF.Back to article page