Figure 3

Summary of Sir2, Hst1, and Sum1 binding across the yeast genome. (a) Gene-based distribution of Sir2, Hst1, and Sum1 binding sites identified with BayesPeak. Filled circles represent relatively small ORFs that were completely embedded within a peak. (b) Composite plots showing that Sir2, Hst1, and Sum1, but not Hst2, are enriched at the most highly expressed genes. The sequence of each annotated ORF in the genome was normalized to 1,000 bins, with another 500 bins upstream and 500 downstream, into which the number of sequencing reads (tags) were distributed. The expression level of each ORF was divided into five quintiles, with the 5th quintile the highest. (c) Venn diagram showing the overlap between genes with Sir2, Hst1 or Sum1 are enriched across >60% of the ORF. **P-value <0.005. (d) Screenshots of Sir2, Hst1, Sum1, and Hst2 enrichment across the PDC1, ENO2, and CDC19 genes. Hst1 acts as a negative control similar to the Input sequence. (e) ChIP assay confirming the enrichment of Sir2, Hst1 and Sum1 across the ORFs of PDC1 and ENO2. Relative IP indicates the ratio of IP PCR signal to the input chromatin PCR signal for each sample. Error bars represent standard deviation.