Figure 1

Summary of PAR-CLIP data of snoRNP core proteins. (A) Profiles of sequencing reads obtained from PAR-CLIP experiments for selected snoRNAs. Black bars in the profiles indicate the number of T→C mutations observed in PAR-CLIP reads at a particular nucleotide. (B) Similarity of binding profiles of core proteins that associate with C/D box snoRNAs. (C) Comparison of protein binding profiles as inferred from RNase T1-treated and MNase-treated PAR-CLIP samples. (D, E) Preferential binding of Fibrillarin to box elements as inferred from PAR-CLIP samples prepared with T1 (D) and MNase ribonucleases (E). (F) Comparison of binding preferences at D'/D box elements and guide regions for snoRNAs with and without a known target. (G) Analysis of binding preferences of Dyskerin for H/ACA box snoRNA-specific elements. D, E, F and G show the cumulative distributions of CLIP read coverage z-scores for nucleotides located in various regions of the snoRNA relative to the overall coverage of the snoRNA. CLIP: cross-linking and immunoprecipitation; MNase: micrococcal nuclease; PAR-CLIP: photoactivatable-ribonucleoside-enhanced cross-linking and immunoprecipitation; snoRNA: small nucleolar RNA; snoRNP: small nucleolar ribonucleoprotein