Table 4 Differential methylation analysis of the eight selected BW-MVPs using the Infinium and DBS data

From: Adult monozygotic twins discordant for intra-uterine growth have indistinguishable genome-wide DNA methylation profiles

		Infinium					DBS
CpG number	Gene	Mean heavy co-twins^a	Mean light co-twins^a	Mean difference	P ^b		Mean heavy co-twins^c	Mean light co-twins^c	Mean difference	P ^b
cg14123607	APBA1	0.22 ± 0.07	0.15 ± 0.07	0.07 ± 0.05	0.0008		0.09 ± 0.05	0.09 ± 0.05	0.006 ± 0.04	0.30
cg12170649	APPL2	0.81 ± 0.04	0.86 ± 0.02	-0.06 ± 0.05	<0.0001		0.97 ± 0.02	0.97 ± 0.02	-0.002 ± 0.02	0.86
cg26404226	NA	0.50 ± 0.05	0.56 ± 0.07	-0.05 ± 0.04	<0.0001		0.35 ± 0.08	0.36 ± 0.06	-0.01 ± 0.05	0.46
cg15487251	IGF2BP2 ^d	0.53 ± 0.05	0.58 ± 0.04	-0.05 ± 0.05	0.002^e		0.65 ± 0.05	0.64 ± 0.06	0.01 ± 0.03	0.04
cg10362113	PAPOLA ^d	0.83 ± 0.08	0.77 ± 0.06	0.06 ± 0.07	0.008		0.98 ± 0.04	0.99 ± 0.03	-0.01 ± 0.05	0.50
cg02409150	PHKG2	0.88 ± 0.05	0.94 ± 0.03	-0.06 ± 0.05	<0.0001		0.93 ± 0.02	0.93 ± 0.02	-0.006 ± 0.03	0.43
cg15049370	PPARGC1B ^d	0.70 ± 0.06	0.77 ± 0.07	-0.07 ± 0.07	0.002^e		0.96 ± 0.01	0.97 ± 0.02	-0.007 ± 0.02	0.23
cg22768222	RUNX2	0.37 ± 0.07	0.31 ± 0.05	0.06 ± 0.07	0.008		0.21 ± 0.03	0.21 ± 0.03	0.001 ± 0.05	1.00

Results of the Wilcoxon signed-rank test of the 8 selected MVPs performed on the Infinium and the DBS data in the 16 MZ twins discordant for birth weight (pair 1 was excluded) are presented. ^aInfinium data are expressed as mean β-value ± SD. ^bHeavy versus light calculated using a Wilcoxon signed-rank test. ^cDBS data are expressed as mean methylation level ± SD, where the methylation level is calculated by dividing the number of reads in which the particular CpG is methylated by the total number of sequenced reads. ^dInfinium and DBS data were adjusted for buccal content (using PTPN7) and the adjusted values are presented. ^eAlso significant without adjusting for buccal marker. Mean difference = Heavy co-twin - Light co-twin. BW-MVP, birth weight-associated methylation variable position; DBS, deep bisulfite sequencing; MZ, monozygotic.

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