TY - JOUR AU - Sasagawa, Yohei AU - Nikaido, Itoshi AU - Hayashi, Tetsutaro AU - Danno, Hiroki AU - Uno, Kenichiro D. AU - Imai, Takeshi AU - Ueda, Hiroki R. PY - 2013 DA - 2013/04/17 TI - Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity JO - Genome Biology SP - 3097 VL - 14 IS - 4 AB - Development of a highly reproducible and sensitive single-cell RNA sequencing (RNA-seq) method would facilitate the understanding of the biological roles and underlying mechanisms of non-genetic cellular heterogeneity. In this study, we report a novel single-cell RNA-seq method called Quartz-Seq that has a simpler protocol and higher reproducibility and sensitivity than existing methods. We show that single-cell Quartz-Seq can quantitatively detect various kinds of non-genetic cellular heterogeneity, and can detect different cell types and different cell-cycle phases of a single cell type. Moreover, this method can comprehensively reveal gene-expression heterogeneity between single cells of the same cell type in the same cell-cycle phase. SN - 1474-760X UR - https://doi.org/10.1186/gb-2013-14-4-r31 DO - 10.1186/gb-2013-14-4-r31 ID - Sasagawa2013 ER -