Phylogeny of P450s. Phylogeny of MPB (Dendroctonus ponderosae, Dpon) P450s with those from the honey bee (Apis mellifera, Amel), silk moth (Bombyx mori, Bmor), and red flour beetle (Tribolium castaneum, Tcas). Red arcs indicate areas of expansion of the mountain pine beetle (MPB) CYP4, and both CYP6 and CYP9 P450 families within the CYP4 and CYP3 clades, respectively. Branches with dots had greater than 80% bootstrap support. The tree was rooted with human (Homo sapiens, Hsap) CYP3A4.