Figure 4

FSH antibody preparation and validation of specificity. (A) Map of FSH protein isoforms indicating location of antigens ID166 and ID173 used for rabbit immunization. BD1: bromodomain 1; BD2: bromodomain 2; CTD: C-terminal domain; ET: extraterminal domain. (B) Nuclear extract from S2-DRSC cells was immunoblotted using FSH-specific antibodies; arrowheads indicate detected FSH isoforms. (C) S2-DRSC cells were depleted from FSH isoforms using RNAi; immunoblotting of corresponding cell lysates using FSH-specific antibodies demonstrates the expected specificity.