NETs that recruit chromosomes to the nuclear periphery have restricted tissue expression. Human tissue blots equally loaded for the tissues listed (20 μg in each lane) were probed with NET antibodies. NETs that had strong chromosome repositioning effects tended to be highly restricted in their expression. In contrast, several other NE proteins and controls were expressed in all tissues (lower panels). Ab1 and Ab2: two commercial antibodies to NET39 revealed restricted tissue expression and the highest levels of expression in muscle, but differed in the reactivity with some other tissues. Asterisk: NET47 migrates at 38 kDa instead of the calculated 46 kDa.