NETs promote movement of partly overlapping and partly distinct sets of chromosomes. (a) Similar to the two-dimensional analysis, an erosion approach was used to divide the three-dimensional nucleus (three-dimensional reconstructions) into shells of equal volume, but six shells instead of the five for the two-dimensional analysis were generated. (b) Quantification of whole chromosome painting in the absence of the array. Mean (± standard deviation) proportion (percentage) of the pixel intensity of human chromosomes 1, 5, 11, 13, and 17 in the two most peripheral and two most internal nuclear shells; n = 100 cells. The results from the three-dimensional analysis (lower panels) recapitulated those from the two-dimensional analysis (upper panels). *P < 0.05 and **P < 0.01, comparing the position of the chromosome in the NET-transfected cells to the NLS-GFP transfected control using Kolmogorov-Smirnov (KS) tests. Statistics for all chromosomes are given in Additional file 1.