miRNA family members show different patterns of tissue-preferential expression and target cleavage. (a) miR166 family-mediated cleavage of Bradi1g13910, which encodes an HD-ZIP III transcription factor. (b) miR156 family-mediated cleavage of Bradi4g33770, which encodes a SPL transcription factor. The schematic diagrams of genomic structures for miRNA target genes are shown. The gray boxes indicate exons. The sequences of miRNA target sites and miRNA family members were aligned. Lines indicate a perfect match. Circles indicate G:U pairs. PARE sequence distribution for target sites are shown with histograms. The expression patterns of miRNA family members were examined for diverse tissues using the splinted ligation-mediated miRNA detection method. Bdi-miR168 was used for the control. bp, base pair; HD-ZIP, homeobox domain-leucine zipper; SPL, Squamosa Promoter Binding Protein-Like; miRNA, microRNA; PARE, parallel analysis of RNA ends; TP10M, transcripts per 10 million.