Figure 6

Correlation between DNA methylation and H3K27me3 mark. (A) During epithelial-mesenchymal transition (EMT), gain of DNA methylation (≥2%) at selected promoters in human mammary epithelial (HMLE) Twist cells cultured either in monolayer (green) or spheres (red) and which were completely unmethylated (≤1%) in HMLE vector cells (blue) is accompanied by gain of the H3K27me3 mark. The x-axis represents distance from the CpG sites gaining DNA methylation to the transcription start sites (TSS). The y-axis represents average intensity of H3K27me3 peaks. (B) During EMT, loss of DNA methylation (≤2%) of selected promoters in HMLE Twist cells cultured either in monolayer (green) or spheres (red) and which were methylated in HMLE vector cells (blue) is not associated with change of H3K27me3 distribution. The x-axis represents distance from the CpG sites losing DNA methylation to the TSSs. The y-axis represents average intensity of H3K27me3 peaks. (C) Distribution of H3K27me3 marks in partially methylated domain (PMDs) in HMLE Twist cells cultured in a monolayer (green) and spheres (red) as compared to HMLE vector cells. (D) Distribution of H3K27me3 marks outside PMDs in HMLE Twist cells either cultured in a monolayer (green) or as spheres (red) as compared to HMLE vector cells.