The evolution, impact and properties of exonic splice enhancers

Table 7 Rate of evolution of ESE and (a) non-ESE sequence and (b) pseudoESE sequence at four-fold degenerate sites

Dataset	Median ESE	Median non-ESE	% Difference	Ptwo-sided
A
ESR	0.3	0.39	-23.1	1.10E-13
INT2.400	0.32	0.36	-11.1	1.10E-13
INT3	0.3	0.36	-16.7	1.10E-13
INT3.400	0.3	0.35	-14.2	1.10E-13
INT3_ESR	0.3	0.35	-14.2	1.10E-13
INT3_ESR_400	0.29	0.35	-17.1	3.40E-13
Ke-ESE	0.35	0.34	2.9	8.20E-08
Ke-ESE400	0.38	0.34	11.8	1.10E-13
PESE	0.31	0.37	-16	1.10E-13
RESCUE	0.32	0.35	-8.5	5.70E-13
B
ESR	0.3	0.35	-14.3	4.50E-13
INT2.400	0.32	0.35	-8.5	4.50E-13
INT3	0.3	0.35	-14.3	4.50E-13
INT3.400	0.3	0.34	-11.8	4.50E-13
INT3_ESR	0.3	0.34	-11.8	4.50E-13
INT3_ESR_400	0.29	0.34	-14.7	2.30E-12
Ke-ESE	0.35	0.35	0	4.10E-06
Ke-ESE400	0.38	0.36	5.6	4.50E-13
PESE	0.31	0.35	-11.4	4.50E-13
RESCUE	0.32	0.35	-8.5	4.50E-13

We consider the proportion of four fold degenerate sites that are changed or unchanged when comparing mouse-human alignments. We split sites by whether in human the sequence matches an ESE or not (Table A) or ESE versus pseudoESE (Table B). We then perform a Wilcoxon paired test considering the rate of evolution of ESE and non-ESE or pseudoESE at each position away from an exon boundary. We also present the % difference between the medians, this being (median for ESE - median for non-ESE)/median for non-ESE. All tests are significant after Bonferonni correction (for consistency the P values are shown in bold).

ISSN: 1474-760X