Summary of TF enrichment within collapsed tissue annotation terms. Enrichment of the fraction of TFs vs. All genes for collapsed annotation terms at each stage-range. Each annotation term is represented as a box, where the y-axis value is the fold enrichment (FE) and the color indicates the stage range. To highlight the organ system trends, collapsed tissue annotations are grouped by organ systems shown on the x-axis. All the annotation terms that did not show significant enrichment (FDR >0.05) are assigned a fold enrichment of zero and placed in the grey area of the plot. For the Ectoderm, VisualPr, CNS, and Foregut organ systems, the positions of the annotation term boxes are adjusted and linked to the actual FE value, represented as a point. Individual collapsed tissue annotation abbreviations and annotation term mappings are detailed in Additional file 3: Table S3. Short key to terms: ME: mesoderm; HM: head mesoderm; TM: trunk mesoderm; HSM: head somatic mesoderm; HVM: head visceral mesoderm: TSM: trunk somatic mesoderm; TVM: trunk visceral mesoderm; FB: fat body; Pl: plasmatocytes; G: garland cells; CM: cardiac mesoderm: CV: cardiovascular system: RG: ring gland; DE: dorsal ectoderm; VE: ventral ectoderm; HEE: head epidermis; DEE: dorsal ectoderm/epidermis; VEE: ventral ectoderm/epidermis; AP: anal pad; O: oenocyte: T: trachea; PS: posterior spiracle; SG: salivary gland; VP: visual primordium; OL: optic lobe; AE: anterior endoderm; PE: posterior endoderm; AMG; anterior midgut: PMG: posterior midgut; MG: midgut; MT: Malpighian tubules; H: hindgut; Ph: pharynx; FG: foregut; AT: atrium; SNS: stomatogastric nervous system; PCE: procephalic ectoderm; CB: central brain: VC: ventral nerve cord; M: midline; P: PNS-photosensory: MS: PNS-mechanosensory; C: PNS-chemosensory; AI: adult imaginal primordium; PC: pole cell; GC: germ cell; GO: gonad; A: amnioserosa: Y: yolk.