Binarized DNA methylation status and pairwise comparison of the CpG sites. (a) Binarized DNA methylation status of the 35 CpG sites showing a stable influence on gene expression over the samples of the primary sarcoma collection. The sarcoma cluster assignment from the initial random forest clustering (Figure 2) is indicated by the colored bars. The CpG short names contain the CpG cluster number together with the importance. Cluster-specific CpG sites are bordered in blue. Hypermethylation and hypomethylation are shown in red and blue, respectively. (b) Pairwise comparison of the 35 preselected CpG sites for the seven sarcoma clusters. One representative CpG site was selected for each comparison (bordered in blue). Significant hyper- and hypomethylation of CpG sites for the two sarcoma clusters are marked in red and green, respectively (Wilcoxon rank sum test). Unchanged CpG sites are indicated in blue. DDLS, dedifferentiated liposarcoma; LMS, leiomyosarcoma; MFS, myxofibrosarcoma; MLS, myxoid liposarcoma; MPNST, malignant peripheral nerve sheath tumor; PLS, pleomorphic liposarcoma; SS, synovial sarcoma; UPS, undifferentiated pleomorphic sarcoma.