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Figure 3 | Genome Biology

Figure 3

From: Transcriptome and proteome quantification of a tumor model provides novel insights into post‐transcriptional gene regulation

Figure 3

Proteome analysis. (A) Optimized mass spectrometry workflow for quantitative proteomics using 4‐plex iTRAQ. (B) Proteome coverage of expressed transcripts. The red line indicates the percentage of quantified proteins in each bin of detected transcripts. Transcripts with FPKM ≥ 1 are considered expressed (dashed gray line, see also Figure 2A). (C) Proteome coverage of different KEGG categories. For each functional category the number of annotated (dark gray), expressed (light gray) and quantified proteins (white) are shown together with the percentage of quantified proteins (red). (D) Box‐and‐whisker plot of protein expression changes between wild‐type and brat samples in different functional categories. FPKM, fragments per kilobase of transcript per million mapped fragments; KEGG, Kyoto Encyclopedia of Genes and Genomes.

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