Figure 2

Steady-state mRNA and polysome-association mRNA profiles of genes expressed in the asexual stage of P. falciparum . (A) Cluster analysis of 4,007 genes based on steady-state mRNA accumulation. (B) Independent cluster analysis of the same set of genes from (A) based on polysome-associated mRNA accumulation. For each cluster, the number of genes (n) and a selection of enriched gene ontology (GO) terms are presented. (C) Number of genes that were found in each combination of steady-state mRNA and polysomal mRNA clusters. Combinations of identical transcription and translation profiles are indicated in dark blue (1,749 genes), while combinations with a partial translational delay are indicated in light blue (738 genes). For 1,280 genes, a delay between the time point of transcript accumulation and the moment of peak translation was observed (shown in light orange). (D) Steady-state mRNA and polysomal mRNA profiles of genes that showed a delay in polysome-association as compared to the time point of transcript accumulation (n = 1,280). Genes are ordered identically in both heatmaps to allow a comparison of steady-state mRNA and polysomal mRNA profiles. To visualize the delay of genes from the mature stages to re-invaded ring stage parasites, the 0 h time point of the next cell cycle is displayed for polysome-associated mRNA. N.a., not available.