Figure 3From: Individual and combined effects of DNA methylation and copy number alterations on miRNA expression in breast tumorsIn -cis miRNAs show functional effects when overexpressed. Breast cancer cell lines were transfected with miRNA mimics (20 nM) and assayed for (a) cell proliferation (Ki67), (b) phosphorylated AKT (p-AKT) levels, (c) cell viability and (d) apoptosis (cleaved PARP (cPARP)), 72Â hours after transfection. The dashed lines indicate cut-off points that were considered significant (see Materials and methods). Asterisks denote significant effects. The data for cell viability are from two replicate experiments with error bars showing standard deviations.Back to article page