Figure 4
Titration to ascertain optimum cell number for use in AHT-ChIP-seq experiments. (A) Proportional Venn diagrams show the overlap of peaks between duplicate experiments for a titration curve of cell number for H3K4me3 in HepG2 and MCF7 cell lines, with highest cell number being log107 and the lowest cell number being log103. No ChIP enrichment was observed for less than 1,000 cells. (B) Line graphs showing percentage peak recovery for titration of cell number, with cell number shown as log10 scale with highest cell number being 10 million.