Promoters and enhancers are associated with fast H3.3 nucleosome turnover whereas gene body and transcription end site regions are associated with slow H3.3 nucleosome turnover. (A) Median and mean turnover indices (TIs) calculated for various genomic regions. Intergenic enhancers were defined as peaks that are located in intergenic regions at least 1 kb from the TSS. Intronic enhancers were defined as peaks located in introns only. Gene body regions were defined as broad peaks that spanned both intronic and exonic regions and shared significant overlap with H3K36me3. (B-E) Distribution profiles of HA-H3.3 from TSS to TES at different time points. (F) Distribution plot illustrating the range of turnover indices within various genomic categories. Turnover rates were log-normalized. RPKM, reads per kilobase of exon model per million reads. (G) Distribution plot illustrating the range of turnover indices of H3.3 nucleosomes within various genomic categories. Log of turnover indices was calculated and re-scaled from 0 to 1.