Distribution and enrichment of H3.3 in the genome. (A) Enrichment of HA-H3.3 at various genomic regions of different annotation. The total read count of each category was normalized over its total length. (B) Pie chart showing the proportions and numbers of peak centers that fall into various genomic categories. (C) Enrichment of HA-H3.3 at various repetitive elements. All_Rep, all repetitive elements; SAT, satellite regions; SINE, short interspersed nuclear element. (D) Count of histone mark peaks that overlap with HA-H3.3 peaks. Overlapped peaks were defined as overlapping when one peak (H3.3 or histone mark) shared 10% of reads with the other. ChIP-Seq libraries for various histone marks were prepared from cells treated with aphidicolin. (E) UCSC Genome browser view illustrating overlap of HA-H3.3 with various histone marks and H2AZ. (F) Distribution profile of HA-H3.3 based on mRNA expression levels as indicated by different colors. RNA-seq libraries were prepared from cells treated with aphidicolin. (G) Distribution profile of HA-H3.1 based on mRNA expression levels as indicated by different colors. ChIP-Seq and RNA-Seq libraries were prepared from untreated (no aphidicolin) cells.