Figure 5

Dynamic rewiring within the co-expression network during differentiation. (A) Heatmap matrix of Spearman correlation analysis of 45 TFs across 120 single-cells at time points 0 h, 1 h, 24 h, and 96 h post differentiation and (B) quantification of significant edges (positive and negative) at each time point. (C) Co-expression networks showing putative relationship between TFs in all time points but highlighting activating or positive correlations (top row: blue edges) and antagonistic or negative correlations (bottom row: red edges) for time points 0 h, 1 h, 24 h, and 96 h post-PMA stimulation (P value <0.01). The node-colors indicate mean gene expression (scaled: blue = low expression; light brown = moderate expression; dark brown = high expression) while the node sizes indicate single-cell gene expression variations (small nodes = low variation; big nodes = high variation).