THP-1 differentiation process. (A) THP-1 cells are stimulated with PMA to induce monocyte/macrophage differentiation 1 day after medium change (mc). Single cells are manually picked at eight defined time points on three independent occasions (n = 120 per each time point). A total of 960 single cells were pre-amplified and profiled against 45 THP1 TFs. (B) Suspended THP-1 cells undergo differentiation upon PMA stimulation and a large majority (>95%) of cells adhere to the dish 96 h post induction with PMA. Scale bar = 50 μm.