A multiple sequence alignment improved with the expert version of Phylo. (a) A section of the input alignment of the P53 gene calculated with MUSCLE. (b) The improved alignment obtained with the expert version of Phylo. Three nucleotides from the elephant sequence (loxAfr3) have been moved to increase the conservation of alignment columns 6, 32 and 33. The player also improved the alignment of columns 48 and 49 and revealed similarities not found in the original alignment. Image produced with Jalview .