Figure 6
From: High-efficiency RNA cloning enables accurate quantification of miRNA expression by deep sequencing
Identification and quantification of the major miR-203 isoforms in skin by deep sequencing. (A) Table showing miR-203 with differential 5′ ends (shown in red). (B) Primer extension for miR-203 using RNA isolated from mouse total epidermis where upper band corresponds to miR-203 and lower corresponds to miR203iso. Dicer conditional knock out skin sample was used as a control. (C) Northern blot probed for miR-203. Left panel shows synthetic miRNA controls, and right panel shows mouse keratinocyte cultures where miR-203 was lowly expressed under the proliferative condition and highly induced in the differentiated condition. Staining of tRNAs by ethidium bromide (EtBr) is shown as the loading control.