Figure 7
FOXM1 regulates genes associated with poor prognosis in ER-positive breast cancer patients. Comparison of the thiostrepton down-regulated genes with a FOXM1 binding site (± 50 kb TSS) with expression data from a breast cancer patient array dataset containing 286 samples (209 ER positive) with patient survival outcome [56] showed a set of 42 probes (38 unique genes) significantly associated with poor prognosis. (a) Heat map showing gene expression in the ER-positive patients. Patients are grouped into good prognosis (non-relapsed, blue ribbon) and poor prognosis (relapsed, red ribbon). Gene expression is represented as light blue or red for patients with expression below or above the gene median, respectively. (b) Kaplan-Meier plot showed a significant positive correlation between patient relapse and expression of genes down-regulated by thiostrepton and associated with FOXM1 binding sites. (c) Kaplan-Meier plot using the same gene signature set as in (b) in an independent breast cancer dataset [57] confirmed correlation with patient survival.