Figure 5

Treatment of MCF7 cells with thiostrepton alters FOXM1 binding genome-wide. (a) Molecular structure of the thiazole antibiotic thiostrepton. (b) Differential binding analysis (DBA) was used to identify significantly (FDR < 0.05) differentially bound peaks in DMSO compared to thiostrepton (TS)-treated cells from four replicates. The dots shown in red represent peaks where FOXM1 binding is significantly increased/decreased compared to the control. Enrichment of Gene Ontology processes associated with the differentially bound peaks was determined using GREAT; the top four significantly enriched processes in the upbound and downbound regions are shown. (c) Heatmap of DBA binding events for two of the replicates showing FOXM1 binding signal intensity in regions up- or down-regulated by thiostrepton treatment in a window of ± 5 kb and total signal intensity of differentially bound peaks showing fold change in binding in thiostrepton-treated compared to control cells. (d) Examples of genomic loci where FOXM1 binding is reduced following treatment of MCF7 cells with thiostrepton. (e) Motif analysis of the 200 bp region around peak center in the differentially bound peaks.