Figure 2

FOXM1 shows co-operative binding with ER in MCF7 cells. (a) Heat map showing overlap between FOXM1 and ER binding events. The window represents ± 5 kb regions centered on FOXM1 binding events. (b) Overlap of binding sites between FOXM1, ER and FOXA1 in MCF7 cells. (c) Motif analysis shown as a wordcloud schematic of the significantly enriched motifs present in regions bound either by both ER and FOXM1 or by FOXM1 only. The text size is inversely proportional to the log P-value. (d) Examples of genomic locations showing regions of joint FOXM1/ER binding and also ER only (PRMT8). (e) Re-ChIP showing co-occupancy of ER and FOXM1 in regions where both factors bind compared with regions where only ER binds (PFKFB3 and PRMT8). (f) Co-immunoprecipitation showing pull-down of ER with FOXM1 antibody.