22G small RNA pathways mediate the lin-35 larval arrest phenotype. (a) Binding profiles at loci encoding candidate small RNA pathway regulatory proteins. (b) qRT-PCR analysis of transcript levels of candidate small RNA pathway regulators in the dissected gonads of wild-type and dpl-1 mutants. Error bars indicate technical replicates. (c) qRT-PCR analysis of transcript levels of candidate small RNA pathway regulators in L1 larvae of wild-type and lin-35 mutants. Error bars indicate biological replicates. (d) Assay of the high temperature larval arrest phenotype of lin-35 mutants at 26°C upon RNAi of candidate small RNA regulators (listed on the x-axis) and empty vector control (L4440).