Figure 3

Tissue-specific target genes in lin-35, efl-1 , and dpl-1 mutants are differentially regulated. (a) Over-representation of tissue-specific candidate gene targets in different published microarray expression datasets. The expression datasets on the x-axis labeled E2F gonad and LIN gonad are from [11], while the 20°C data sets are from [20], and the 26°C data sets are from [10]. Statistically significant deviations are indicated by asterisks (P < 1.0e-05, hypergeometric probability test). (b) qRT-PCR results for selected germline-specific candidate target genes that were not considered regulated in the gonad microarray datasets. Fold difference of expression was compared between control (unc-4) and mutant (unc-4 dpl-1) RNA from dissected gonads, and normalized to hexokinase expression. Error bars indicate technical replicates. The dashed line indicates 1.5-fold difference. (c) qRT-PCR results for selected soma-specific (blue), intestine-specific (orange), and broadly bound (black) candidate target genes that did not show any regulation in the 20°C or 26°C L1 microarray datasets. The fold difference of expression of each gene was compared between wild-type (N2) and lin-35(n745) mutant L1s raised at 26°C, and normalized to actin (act-3) expression. Error bars indicate technical replicates. The dashed line indicates 1.5-fold difference.