LIN-35 exhibits reduced binding in the germline. (a) Example of binding across the entirety of chromosome I for each factor in the germline (indicated by the promoter, either Ppie-1, red, or Pmex-5, green), and for LIN-35 in the soma (Plin-35, blue) and the intestine (Pges-1, orange). Three individual genes are shown below, two of which are germline-specific and show little (left) or no (middle) LIN-35 binding, and one that is broadly bound and shows LIN-35 binding in somatic tissues and not in the germline. (b) Venn diagram comparing overlap of binding sites between Pmex-5:LIN-35:GFP and Ppie-1:EFL-1:GFP. (c) In situ hybridization of candidate germline-specific genes (par-3 and rme-2) in wild-type (wt) and lin-35 gonads. AS, antisense probe; S, sense probe. Distal tip marked by an asterisk. Scale bars = 50 μm.