Inflammation-associated enterotypes, host genotype, cage and inter-individual effects drive gut microbiota variation in common laboratory mice

Table 1 PERMANOVA test for significance of factors contributing to overall differences in microbiota composition

	Phylum	Class	Family	Genus	OTU
Genotype	2.00E-07	2.00E-07	2.00E-07	2.00E-07	2.00E-07
Cage	0.0238	5.08E-05	7.26E-05	6.20E-06	2.00E-07
Sex	0.44	0.2972	0.3003	0.3926	0.2456
Sex_block	0.374	0.09805	0.1091	0.2072	0.05531

Genotype and cage were significantly associated with differences in microbiota composition whereas sex did not have an effect. Note that only B6 mice were used to test for sex effects as we only had females and males from B6. We repeated the PERMANOVA test for the complete dataset, using genotype in a blocked design to test for sex effects (sex_block). We used 5 × 10⁷ permutations to calculate the significances.

ISSN: 1474-760X