Figure 4
Cause and effect in the relationship between pH c and growth rate. (a) Wild-type pHc plotted against growth rate μ. Dots represent the time course data of 24 independent biological replicates of full 16 hour growth curves. The data were fit to an exponential dependence of μ on pHc (Additional file 1). Grey dots represent points from the first 1.5 hours after inoculation into fresh medium. (b-d) Titration of pHc sets growth rate. Strain pma1-007 was cultivated in unbuffered, pHex controlled batch fermentors, and titrated to pHex values indicated using strong acid (HCl). Figures represent pHc (b), growth (c), and pHc plotted against growth rate μ (d). Data points represent the average of 12 technical replicates, error bars represent standard deviations.