Prediction of metabolic flux distributions in knockout mutants of E. coli, S. cerevisiae , and B. subtilis . (a) E. coli, (b) S. cerevisiae, (c) B. subtilis. The sum of squared errors per flux (SSE) is shown for each mutant strain, and the average SSE across all mutants is shown for each organism. The RELATCH predictions were made using the relaxed parameter values for (a) and the tight parameter values for (b,c) since the strains were grown in a chemostat and batch cultures, respectively. PP, pentose phosphate, TCA, tricarboxylic acid cycle.