Figure 3

TCF7L2 binding sites are distal and enriched for active enhancer histone marks. (a) Shown for the TCF7L2 binding sites in the six cell types and for the 1,864 peaks commonly bound in all six cells is the percentage of TCF7L2 binding sites in different genomic regions (hg19) relative to the nearest transcription start site (TSS). (b) The percentage of active enhancer regions containing a TCF7L2 binding site; active enhancers were defined by taking the regions that have an overlap of H3K4me1 and H3K27ac ChIP-seq peaks for the given cell line. (c) Heatmaps of the ChIP-Seq tags for H3K27ac and H3K4me1 at TCF7L2-bound regions (±3 kb windows around the center of all TCF7L2 peaks) for each cell line were generated by k-means cluster analysis. (d) The average RNA polymerase II and histone modification profiles of MCF7 cells are shown for the ±3 kb windows around the center of TCF7L2 peaks identified as proximal to RefSeq genes (upper graph) or distal to RefSeq genes (lower graph).