A computational framework for boosting confidence in high-throughput protein-protein interaction datasets

Table 1 Comparison of overlaps achieved by Braun et al. and our method when some of the initial Y2H interaction pairs are re-tested using LUMIER assay

Yeast strains implementation	Number validated (LUMIER)	Y2H PPIs	Percentage overlap
Y strain 2 m 1 reporter 1 mM_3-AT (Braun et al.)	19	33	57
Y strain 2 m 2 reporters 1 mM_3-AT (Braun et al.)	13	22	59
Y strain CEN 1 reporter 1 mM_3-AT (Braun et al.)	17	23	74
MaV CEN 2 reporters 20 mM_3-AT (Braun et al.)	9	14	64
Our prediction	14	19	74
Our prediction^a	10	11	91

ISSN: 1474-760X