Observed genetic interactions between S. pombe genes support degree predictions. (a) Model predictions were validated on a second, whole-genome set of interaction screens in S. pombe that are independent of the training data. Eight query deletion mutants were crossed with the entire non-essential deletion collection in S. pombe. In total, genetic interaction (epsilon) scores were measured for approximately 23,000 gene pairs. Epsilon scores are tightly centered at 0, thus interactions called for scores of ± 0.08 or more extreme are rare. (b) The collection of non-essential S. pombe genes (n = 2,907) were grouped by the number of interactions each has with the eight query genes for which full-genome screens were performed. Numbers in parentheses give the number of genes for which this degree was observed. For each degree, the box plot shows the distribution of predicted degrees, which are expressed as percentiles. There is a strong positive correlation (Pearson's r = 0.40, P < 10-111) between predicted and actual degree.