Sketch of the central metabolism and localization of mutations in C. glutamicum together with whole-genome sequencing results of the mutant K051. (a) Pathways and reactions required for and related to lysine synthesis. Genes that carry mutations leading to amino acid exchanges are indicated as follows: blue box, mutations known previously; yellow box, new mutations identified by targeted sequencing in 40 mutants; orange box, new mutations derived from the K051 genome. (b) Localization of the 268 SNPs in the genome of strain K051 determined by whole-genome sequencing. The size of the genome is 3.28 Mb, scale on the left. The mutations are classified into those causing an amino acid exchange, those that are silent, those leading to a stop codon, and those located in non-coding regions. The genome sequence of strain K051 is deposited at the European Nucleotide Archive under the accession number HE802067.