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Figure 1 | Genome Biology

Figure 1

From: A systematic screen reveals new elements acting at the G2/M cell cycle control

Figure 1

Identification of small size mutants. (a) Strategy for the identification of small size mutants in the fission yeast deletion collection. Microcolonies of 2,969 strains carrying individual deletions of different non-essential genes were visually screened on agar plates of yeast extract complex medium (YE4S). Candidate strains were grown in YE4S liquid exponential culture and their cell size determined in a secondary visual screen. Cell length and width of septated cells were measured for each of the selected strains. Gene deletions were confirmed by PCR, and co-segregation of the phenotype with the gene marker used in the deletion was checked. (b) Cell length at division of the small size mutants growing exponentially in complex medium. Boxes enclose 50% of the data and lines within the box show median cell length. Whiskers mark maximum and minimum values that fall within 1.5 standard deviations. Values outside this range are displayed as dots (n = 60 cells). Wt, wild type. (c) Dividing cells carrying additive combinations of mutations identified in the screen. Cell wall was stained with Blankophor and cell length at division in microns is indicated under each panel. (d) Length of cells carrying empty vector and overexpressing snf5 or ski3. Left panels: cell length distribution of the whole population after 20 h of overexpression. Values correspond to the average cell length in microns of the whole population and standard deviation (n > 125 cells). Right panels: Blankophor-stained cells after 20 h of overexpression.

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