Mammalian tissues defective in nonsense-mediated mRNA decay display highly aberrant splicing patterns

Table 1 Splice events and PTC+ classification

Tissue	Splice event	Total events	PTC+ events	Percentage PTC+	Exclusion events	PTC upon exclusion	Inclusion events	PTC upon inclusion
BMM	Total ES	730	281	38%	428	201	302	80
	Single ES	511			310	148	201	80
	Multiple ES	219			118	53	101	-
	A5SS	130	68	52%
	A3SS	238	110	46%
	MXE	44	5	11%
	AFE	130	44	34%
	ALE	49	NA	NA
Liver	Total ES	3,102	1,285	41%	1,926	965	1,176	320
	Single ES	1,505			932	531	573	320
	Multiple ES	1,597			994	434	603	-
	A5SS	449	263	59%
	A3SS	654	370	57%
	MXE	475	66	14%
	AFE	172	66	38%
	ALE	143	NA	NA

Splice isoform classes are shown for both single and multiple exon skipping (ES), alternative 5' splice site (A5SS), alternative 3' splice site (A3SS), mutually exclusive exons (MXE), alternative first exon (AFE) and alternative last exon (ALE). For ALE, no events were classified as PTC+, since the class relies on splicing to two different last exons. Here, a ΔPSI > 20% was required for all classes. NA, not applicable.

ISSN: 1474-760X