Expression of the NAT transcripts is increased in dcl1 - 7 fwf2 and dcl3 - 1 mutants. Expression was examined by quantitative RT-PCR and Actin2 was used as an internal control. Total RNA (5 μg) was treated with DNaseI and then subjected to reverse transcription. Error bars indicate standard deviations derived from three technical replicates. Similar results were obtained from two biological replicates.