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Figure 3 | Genome Biology

Figure 3

From: Defining the molecular profile of planarian pluripotent stem cells using a combinatorial RNA-seq, RNA interference and irradiation approach

Figure 3

Smed-H2B RNAi does not affect differentiated cell types and tissues. (a-f) WMISH of the neoblast markers Smedwi-1 and Smedwi-2 (also expressed in the CNS) (a), the nervous system markers h.10.2f and Smed-cintillo (arrows) (b), the digestive system markers Smed-laminin and Smed-porcn-1 (c), the protonephridial markers Smed-CAVII-1 and Smed-innexin-10 (d), the secretory cell markers Smed-mag-1 and Smed-tcen49 (e) and the body positional markers Smed-slit and Smed-ifb (f) in control(RNAi) (top panels) and Smed-H2B(RNAi) (bottom panels) animals 5 days after RNAi. Smed-H2B(RNAi) animals show a dramatic decrease of Smedwi-1 and Smedwi-2 signals 5 days after RNAi (a), but no significant difference for any differentiated cell marker (b-f). Anterior is to the left. Scale bars: 500 μm.

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Genome Biology

ISSN: 1474-760X

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