Figure 10

NB-expressed transcripts reveal the molecular detail of morphological observations from NBs. (a-d) Smed-H2B(RNAi) IS versus irradiation IS plot of the transcripts compiled with the GO term 'nucleus' (a, blue), the GO terms 'chromosome', 'chromatin binding' and 'chromosome organization' (b, green), the presence of RNA recognition motif, DEAD/DEAH helicase, Tudor and PIWI/PAZ domains (c, yellow), and the KEGG pathways 'DNA replication' and 'Cell cycle' (d, red). Green horizontal lines represent the median Smed-H2B(RNAi) IS of each compilation. Our list of NB-expressed transcripts is enriched in nuclear components (a), chromatin associated components (b), RNA binding and putative RNA granule components (c) and cell division machinery (d). Nuclear components (a) and chromatin-associated components (b) tend to be distributed all over the plot. However, putative CB components (c) tend to be distributed in the upper part of the plot, suggesting expression outside of the NB compartment, while cell division machinery components (d) tend to be distributed close to the axis, indicating their expression in NBs exclusively.