Figure 1

Smed-H2B is expressed in NBs but not in the CNS. (a) WMISH of Smed-H2B, showing the characteristic expression pattern of genes expressed in NBs. (b-d) Sagital section of a FISH experiment on histological sections of Smed-H2B (c), counterstained with nuclei staining (b), and a merged image (d). Smed-H2B-positive cells are distributed in the parenchyma of the animal, absent from the pharynx (ph), the most anterior tip of the head, gut (g), and brain (b), consistent with its expression in NBs. (e-j) WMISH of Smed-H2B (e,f), Smedwi-1 (g,h) and Smedtud-1 (i,j) in non-irradiated (e,g,i) and irradiated specimens (f,h,j). All the Smed-H2B signals disappear after irradiation, similar to Smedwi-1 and unlike Smedtud-1, which retains prominent expression in the planarian CNS. (k-m) Detail of a sagital section of a FISH experiment on histological sections of Smed-H2B (l), counterstained with nuclei staining (k), and a merged image (m). No expression of Smed-H2B is detected in brain cells. b, brain, g, gut; e, eye; ph, pharynx. Anterior is to the left in all panels. Scale bars: 500 μm (a-j); 50 μm (k-m).