Chromatin structural changes around satellite repeats on the female sex chromosome in Schistosoma mansoni and their possible role in sex chromosome emergence

Table 3 Antibodies used for native ChIP (N-ChIP)

Antibody	Host	Product	Lot	Saturating quantity used for N-ChIP^a
H3K9ac	Rabbit	Upstate, 07-352	DAM16924924	8 µl
H3K4me3	Rabbit	Upstate, 04-745	NG1680351	4 µl
H3K9me3	Rabbit	Abcam, Ab8898	733951	4 µl
H3K27me3	Rabbit	Diagenode, pAb-069-050	A29900242	8 µl

^aSaturating quantities for H3K9ac and H3K9me3 antibodies were previously determined [56]. Saturating quantities for H3K4me3 and H3K27me3 were determined in this study by a titration experiment (data not shown).

ISSN: 1474-760X