Comparison of per-mapped-base error rates. Percentage per-mapped-base error rates for the Bustard, Ibis and AYB base callers compared over several sets of reads of varying read length and chemistry versions. The height of the bars are the percentage of bases that differ from the reference genome, conditioned on reads having been mapped with the stated criteria (a total of 1, . . . , 5 edits relative to the reference). Ibis failed to process the second end of the HiSeq data so no bars are shown.