Figure 3

miR-29 repression of extracellular matrix protein production with quiescence. (A) Gene expression changes induced 48 h after miR-29 transfection into fibroblasts. The x-axis denotes the mean log₂ fold change in expression compared to negative control, and the y-axis denotes -log₁₀ of the P value of a one-sided t-test. (B) Empirical cumulative distribution function of log₂ fold-changes induced by miR-29 transfection, comparing predicted targets to all other non-target genes. (C) Quiescence microarray expression timecourses (Figure 2A) of each miR-29 target in Table 1 (shown in gray), along with the mean log₂ fold change at each timepoint (shown in red). (D) Protein expression, as determined by immunoblotting, of selected miR-29 targets in proliferating (P), mitogen-starved (MS), or contact inhibited (CI) states with transfection of a negative control microRNA or miR-29. Collagen III here appears as a doublet corresponding to its two isomers. Immunoblots to GAPDH and α-Tubulin are shown as examples of genes not targeted by miR-29 and as loading controls.