Figure 2

Changes in target genes with quiescence. (A) Hierarchical clustered heat map representing the log₂ fold change of gene expression for all 15,560 consistently detectable genes during 1, 2, 4, 8, 24, and 96 h of serum starvation (SS), 1, 2, 4, 8, 24, and 48 h serum restimulation (SR), and 7 and 14 days (each repeated twice) of contact inhibition (CI). Expression in serum starvation and contact inhibition is shown relative to proliferating cells, and expression during serum restimulation is shown relative to 4-day serum-starved cells. Colors are as in Figure 1A. Numerals designate 4 different clusters chosen from the hierarchical clustering tree. Select enriched gene ontology terms for each of the clusters are shown in Additional File 1, Table S2. (B) Volcano plot of the mean projection of the microRNA target genes' log₂ expression onto the array's first eigengene (Additional File 1, Figure S3B) on the x-axis versus the log₁₀ P value of the mean projection on the y-axis.