Parental inheritance of H3K27me3 in reciprocal F1 hybrids. (a) Venn diagram comparing H3K27me3 targets identified in Col × Ler and Ler × Col F1 hybrids by ChIP-Seq. (b) Venn diagram comparing H3K27me3 targets identified in Col by ChIP-chip and in Col × Ler F1 hybrids by ChIP-Seq. (c) The H3K27me3 enrichment of four Ler-specific H3K27me3 targets in Col (light gray), Ler (dark gray), Col × Ler (white) and Ler × Col (black) F1 hybrids measured by ChIP qPCR. The enrichment was calculated relative to the enrichment at FLOWERING LOCUS T. Error bars represent standard error of one representative experiment. (d) Allele-specific H3K27me3 in reciprocal hybrids of Col and Ler. Twenty-two SNPs for 12 Ler-specific H3K27me3 targets in the Ler × Col F1 hybrids and 35 SNPs for 15 Ler-specific Col × Ler F1 hybrids were used for allele frequency calculation. The dashed lines show the value of 0.5, which is the expected allele frequency value for heterozygous genes if both alleles are equally H3K27 tri-methylated.